General Cell Based Assays
Cayman Chemical CellBased Assay Buffer Tablet
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Cayman Chemical Cysteinyl Leukotriene Express
Cayman’s CysLT Express ELISA Kit is a competitive assay that can be used for quantification of CysLTs in urine, plasma, serum, lavage fluids and aspirates, culture media, and other sample matrices. The assay has a range of 7.8-1,000 pg/ml, an average sensitivity (80% B/B0) of 24 pg/ml, and a lower limit of detection (LLOD) of 6.4 pg/ml. NOTE: This kit measures all CysLTs. For measurement of specific CysLTs, please see Cayman Chemical’s Leukotriene E4 ELISA Kit (Item No. 501060) and Leukotriene C4 ELISA Kit (Item No. 501070).
Cayman Chemical Urea FluorometrIc asy KIt 96
Urea is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays a role in the countercurrent exchange system of the nephrons, allowing for re-absorption of water and critical ions from the excreted urine. Cayman’s Urea Fluorometric Assay provides a convenient method for detecting urea in plasma, serum, and urine. In this assay, urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Ammonia reacts with the detector resulting in a fluorescent product. Fluorescence is analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.
Cayman Chemical FFA asy Cofactr MIxture 1 1ea
A menin-MLL1 protein-protein interaction inhibitor binds to menin (Kd 22 nM) and inhibits the interaction between menin and MLL1 in a cell-free assay (IC50 46 nM) reduces cell growth (GI50 3 UM) and induces cell cycle arrest at the G1 phase in MV4-11 cells
Cayman Chemical DIrect BIlIrubIn Buffer 8mL
Direct bilirubin buffer has been tested and formulated to work exclusively with Cayman’s Total and Direct Bilirubin Colorimetric Assay Kit (Item No. 701720).
Eurofins Discoverx InCELL Hunter eXpress HCK Kinase Binding Assay, 2pl Kit. (96-0041E15CP0M)
The InCELL Hunter eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cell line included overexpresses HCK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Epigentek Inc Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) (96 assays)
The Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) is a complete set of optimized buffers and reagents for measuring activity/inhibition of total cytosine oxygenase TET enzymes in nuclear extracts or purified TET isoforms (TETs 1-3) from a broad range of species such as mammals, plants, fungi, and bacteria, and in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.
Hampton Research CRYSTALHARP PLATE (SWIS 1EACR
HR3-188/CrystalHarp Plate (Swissci) - each. Please note items from this supplier are non-returnable.